General Microbiology — MCQs Biology 50 min Score: 0 Attempted: 0/50 Subscribe 1. Microbiology is the study of: (A) Animals only (B) Plants only (C) Microorganisms (D) Fungi only 2. Bacteria are classified as: (A) Eukaryotes (B) Prokaryotes (C) Fungi (D) Viruses 3. The study of viruses is called: (A) Phycology (B) Mycology (C) Bacteriology (D) Virology 4. Fungi are: (A) Algae (B) Prokaryotic organisms (C) Viruses (D) Eukaryotic organisms 5. The scientist who first observed microorganisms was: (A) Alexander Fleming (B) Louis Pasteur (C) Robert Koch (D) Antonie van Leeuwenhoek 6. Bacterial cell wall contains: (A) Cellulose (B) Peptidoglycan (C) Chitin (D) Starch 7. Gram-positive bacteria stain: (A) Yellow (B) Red (C) Green (D) Purple 8. Gram-negative bacteria stain: (A) Purple (B) Pink/Red (C) Blue (D) Brown 9. Bacteria reproduce mainly by: (A) Binary fission (B) Budding (C) Spore formation (D) Conjugation only 10. Endospores are: (A) Resistant structures formed by some bacteria (B) Only reproductive cells (C) Only nutrient cells (D) Only protein structures 11. The medium used to grow bacteria is called: (A) Culture medium (B) Soil (C) Water (D) Air 12. Pure culture contains: (A) Multiple types (B) Only one type of microorganism (C) Only viruses (D) Only fungi 13. The streak plate method is used for: (A) Counting bacteria (B) Isolating pure colonies (C) Staining bacteria (D) Observing motility 14. Aseptic technique prevents: (A) Staining (B) Growth (C) Contamination (D) Motility 15. Louis Pasteur is known for: (A) Staining techniques (B) Discovery of penicillin (C) Germ theory of disease (D) Pasteurization 16. Robert Koch is famous for: (A) Pasteurization (B) Vaccination development (C) Postulates proving microorganisms cause disease (D) Antibiotic discovery 17. Bacterial shapes include: (A) Cocci, bacilli, spirilla (B) Only cocci (C) Only spirilla (D) Only bacilli 18. Cocci are: (A) Spiral bacteria (B) Rod-shaped bacteria (C) Spherical bacteria (D) Filamentous bacteria 19. Bacilli are: (A) Spiral bacteria (B) Spherical bacteria (C) Rod-shaped bacteria (D) Star-shaped bacteria 20. Spirilla are: (A) Filamentous bacteria (B) Spherical bacteria (C) Rod-shaped bacteria (D) Spiral-shaped bacteria 21. Bacterial motility is facilitated by: (A) Cilia (B) Flagella (C) Pseudopodia (D) Pili only 22. Bacterial pili are used for: (A) Movement only (B) Conjugation and attachment (C) Nutrition only (D) Spore formation 23. Facultative anaerobes: (A) Grow without oxygen only (B) Require oxygen only (C) Can grow with or without oxygen (D) Are non-living 24. Obligate anaerobes: (A) Are fungi only (B) Require oxygen (C) Can tolerate both (D) Cannot survive in the presence of oxygen 25. Obligate aerobes: (A) Require oxygen to grow (B) Do not need oxygen (C) Can grow with or without oxygen (D) Are viruses only 26. Autotrophic bacteria obtain energy from: (A) Air only (B) Organic food only (C) Water only (D) Sunlight (photoautotrophs) or chemicals (chemoautotrophs) 27. Heterotrophic bacteria obtain energy from: (A) Organic matter (B) Sunlight (C) Minerals (D) Air only 28. Antibiotics are produced by: (A) Animals only (B) Plants only (C) Microorganisms like bacteria and fungi (D) Viruses only 29. Penicillin is produced by: (A) E. coli bacteria (B) Streptococcus bacteria (C) Penicillium fungi (D) Aspergillus fungi 30. Microbial fermentation is used in: (A) Only sugar refining (B) Only meat production (C) Only soap production (D) Yogurt, cheese, and alcohol production 31. Lactic acid bacteria are used in: (A) Antibiotic production only (B) Beer only (C) Yogurt and cheese production (D) Vinegar production only 32. Microorganisms that can survive extreme conditions are called: (A) Pathogens (B) Extremophiles (C) Commensals (D) Saprophytes 33. Thermophiles grow best at: (A) High temperatures (B) Low temperatures (C) Neutral temperatures (D) Only in air 34. Psychrophiles grow best at: (A) High temperatures (B) Low temperatures (C) Neutral temperatures (D) Only in soil 35. Halophiles grow best in: (A) Acidic soil only (B) Low salt (C) Freshwater only (D) High salt concentrations 36. Microbial pathogens cause: (A) Water purification only (B) Plant growth (C) Photosynthesis (D) Infectious diseases 37. Endotoxins are: (A) Toxins produced by fungi (B) Toxins released from bacterial cell walls (C) Toxins produced by viruses (D) Toxins in water only 38. Exotoxins are: (A) Toxins in plants only (B) Toxins inside spores (C) Toxins in soil (D) Toxins secreted by bacteria 39. Microbial biofilms are: (A) Free-floating microbes only (B) Communities of microorganisms attached to surfaces (C) Viruses only (D) Fungi only 40. Koch’s postulates are used to: (A) Identify plants (B) Establish the causative agent of disease (C) Produce antibiotics (D) Ferment milk 41. Normal microbiota refers to: (A) Viruses only (B) Pathogens only (C) Fungi only (D) Microorganisms naturally present in the body 42. Microorganisms used in sewage treatment are called: (A) Algae only (B) Pathogens only (C) Decomposers (D) Protozoa only 43. Bacteriophages are: (A) Viruses that infect humans only (B) Viruses that infect bacteria (C) Fungi (D) Algae 44. Microbial transformation of organic matter into methane is called: (A) Nitrification (B) Fermentation (C) Photosynthesis (D) Methanogenesis 45. Antibiotic resistance occurs due to: (A) Genetic mutations and misuse of antibiotics (B) Proper antibiotic use only (C) Vaccination only (D) Filtration only 46. Aseptic techniques include: (A) Only using gloves (B) Only washing hands (C) Only feeding microbes (D) Sterilization of tools and working under clean conditions 47. Anaerobic bacteria grow in: (A) Both oxygen and air (B) Presence of oxygen only (C) Absence of oxygen (D) Only sunlight 48. Microbial nitrogen fixation converts: (A) Ammonia into nitrogen only (B) Atmospheric nitrogen into ammonia (C) Nitrogen into nitrate only (D) Proteins into amino acids 49. Microbes in biotechnology are used for: (A) Only water filtration (B) Only soil formation (C) Only plant growth (D) Antibiotics, enzymes, vaccines, and fermentation 50. General microbiology is important for: (A) Only agriculture (B) Medicine, industry, agriculture, and environment (C) Only industry (D) Only medicine